Human TFF1 PicoKine™ ELISA Kit 试剂盒

¥2,000.00

SKU: EK1232 分类:
ek1232-human-tff1-picokine-elisa-kit-standard-curve
产品货号: EK1232
应用: ELISA
敏感性: <10pg/ml
样品种类: cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

产品描述

技术细节
试剂盒内容
指标信息
应用数据和图片
文献和用户反馈
常见用户提问

产品概况

产品名称 Human TFF1 PicoKine™ ELISA Kit
产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TFF1
引用此产品 Human TFF1 PicoKine™ ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog #EK1232
验证种属 Human
免疫原 标准品表达系统:Expression system for standard: E.coli; Immunogen sequence: E25-F84
交叉反应 There is no detectable cross-reactivity with other relevant proteins.
规格 96wells/kit

产品性质

敏感性 <10pg/ml
*敏感性/最低检测浓度计算方式:敏感性等于20个空白对照孔的平均O.D.值加上2个标准差后对应的浓度。样品的O.D.读数只有在高过敏感性指标的时候才具有统计学显著性。
检测范围 15.6pg/ml-1000pg/ml*检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
样品类型 cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
包被抗体 monoclonal antibody from mouse|polyclonal antibody from goat
检测抗体
一般表达浓度 以下文章提供了TFF1的表达量的信息:

保存条件 4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)

试剂盒内容

SKU 名称 数量
Catalog number Description Quantity
96-well plate precoated with anti- human TFF1 antibody 1
ST0000-1 lyophilized recombinant human TFF1 standard 1ng/tubex2
AR1107 biotinylated anti- human TFF1 antibody 130ul(dilution 1:100)
AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
AR1106-1 sample diluent buffer 30 ml
AR1106-2 antibody diluent buffer 12ml
AR1106-3 ABC diluent buffer 12ml
AR1104 TMB color developing agent 10ml
AR1105 TMB stop solution 10ml
Adhesive cover 4

*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

  • 1. 96孔读板仪器。
  • 2. 自动洗板机.
  • 3. 移液器和枪头。建议使用多通道移液器/排枪。
  • 4. Eppendorf 管。
  • 5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
    • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

指标相关信息 (来源: Uniprot.org)

小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。

基因名 TFF1
蛋白名 Trefoil factor 1
基因全名 Trefoil factor 1
蛋白功能 Stabilizer of the mucous gel overlying thegastrointestinal mucosa that provides a physical barrier againstvarious noxious agents. May inhibit the growth of calcium oxalatecrystals in urine..
Tissue Specificity Found in stomach, with highest levels in theupper gastric mucosal cells (at protein level). Detected in gobletcells of the small and large intestine and rectum, smallsubmucosal glands in the esophagus, mucous acini of the sublingualgland, submucosal glands of the trachea, and epithelial cellslining the exocrine pancreatic ducts but not in the remainder ofthe pancreas (at protein level). Scattered expression is detectedin the epithelial cells of the gallbladder and submucosal glandsof the vagina, and weak expression is observed in the bronchialgoblet cells of the pseudostratified epithelia in the respiratorysystem (at protein level). Detected in urine (at protein level).Strongly expressed in breast cancer but at low levels in normalmammary tissue. It is regulated by estrogen in MCF-7 cells. Strongexpression found in normal gastric mucosa and in the regenerativetissues surrounding ulcerous lesions of gastrointestinal tract,but lower expression found in gastric cancer (at protein level)..
Sequence Similarities Contains 1 P-type (trefoil) domain.
Subcellular Localization Secreted.
Uniprot ID TFF1: P04155
指标别名 Trefoil factor 1; Breast cancer estrogen-inducible protein; PNR-2; Polypeptide P1.A; hP1.A; Protein pS2; TFF1; BCEI, PS2;
相关研究领域 signal transduction|signaling pathway|nuclear signaling|nuclear hormone receptors|estrogen

Trefoil factor 1指标简介

TFF1(Trefoil factor 1), also known as pS2, is a protein that in humans is encoded by the TFF1 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. It is found that TFF1 in normal human urine inhibited the growth of calcium oxalate crystals. Urinary TFF1 showed an inhibitory potency similar to that of nephrocalcin, and inhibition was dose dependent and inhibited by TFF1 antisera, particularly by antisera directed to the TFF1 C terminus. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic calcium oxalate kidney stones were significantly less than those found in controls. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.

Human TFF1 PicoKine™ ELISA Kit 图片

点击图片放大

Human TFF1 PicoKine ELISA Kit standard curve

批内差/批间差

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 16 16 16 24 24 24
Mean(pg/ml) 92 332 585 103 310 605
Standard deviation 3.5 14.94 32.76 4.84 16.43 38.72
CV(%) 3.8 4.5 5.6 4.7 5.3 6.4

Human TFF1 PicoKine™ ELISA Kit的典型数据示例(取自最近的一批次质检数据)

(TMB 孵育条件: 37°C, 25-30min)

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000
O.D. 0.015 0.042 0.066 0.110 0.231 0.457 1.017 2.396

*典型数据仅供参考。用户需要自己建立标准曲线。