Application details contain suggested dilutions. End user should optimize the final working concentrations.
You can check the tissue specificity below for information on selecting positive and negative control.
Some Boster’s antibodies are supplied in the presence of sodium azide. Low levels (0.02 -0.05% w/v) of sodium azide are used to prevent microbial contamination. A simple buffer exchange column is recommended to anyone who may be using Boster’s antibodies to, 1) treat or stain live cells, or 2) perform any primary amine coupling reaction.
All conjugated antibodies should be stored in light-protected vials or covered with a light pro-tecting materials (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 month at 4℃. if longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20 to -80℃. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
If glycerol will not interrupt downstream applications, the addition of glycerol to 50% (vol-ume/volume) is a commonly used cryoprotectant. Store antibodies with glycerol at -20 ℃, not -80℃.
BOSTER BIOLOGICAL TECHNOLOGY
3942 B Valley Ave,Pleasanton,CA 94566
Web: www.bosterbio.com Phone: 888-466-3604
Fax: 925-215-2184 Email: firstname.lastname@example.org